Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Electron Microscopy Sciences Tris (Hydroxymethyl) Aminomethane Free Base, Reagent 100 GR
CAS #77-86-1 C₄H₁₁NO₃ F.W. 121.14
Assay: >99.9%
White crystalline powder. A 40% (w/v) aqueous solution. Hydroxymethyl Aminomethane.
Grade: Reagent
eMolecules 2167889-81-6 | tert-butyl 4-(hydroxymethyl)-2,2-dimethyl-piperidine-1-carboxylate | MFCD30497054 | 1g
Medchem Express | NSC-70220 | 5mg | 495799510 | HY-101796 | 4551-00-2 | 325.367 | C22H15NO2
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Electron Microscopy Sciences Tris Buffered Saline, pH 7.4 500 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 7.4
![Research Products International Corp TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60010-1000.021.JPG-250.jpg)
Research Products International Corp TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 1 Liter
TAE 10X Solution is a concentrated Tris-acetate-EDTA buffer used in molecular biology for DNA and RNA electrophoresis. When diluted to 1X, it maintains pH (~8.3) and low ionic strength. It supports clear nucleic acid separation, extended run times, and protects samples from degradation by chelating divalent metal ions like Mg²⁺.
Substance Name: [TRIS Acetate EDTA 10X Solution]
10X solution contains:
TRIS-Base: 0.4 M
Acetic Acid: 0.2 M
EDTA: 10 mM
pH (10X Solution (@25°C): 8.2-8.4
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-20 Bis-Tris Preca
mPAGE 4-20% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Teknova 2.5M Tris-HCl pH 7.5. Endotox
2.5M Tris-HCl, pH 7.5. Endotoxin Tested. 1000mL, Sterile. 6 pack.
Medchemexpress LLC Tris buffered saline | 10 TABLETS
Tris buffered saline is supplied as ready-to-dissolve tablets for preparing Tris/NaCl buffer solutions commonly used in molecular biology and protein applications. The tablet format simplifies buffer preparation and storage while delivering a stable pH environment for assays and electrophoresis.
- Ready-to-use tablet format for convenient buffer preparation.
- One tablet dissolves in 15 mL deionized water to yield 0.05 M Tris and 0.15 M sodium chloride.
- pH approximately 7.6 at 25 °C.
- White to off-white solid tablets for easy visual quality check.
- Suitable for DNA/RNA extraction, PCR, and protein electrophoresis.
- Compact packaging reduces storage space and eases transport.
Biotang Inc TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml
TE BUFFER, pH 8.0 autoclaved. Ready to use, 250 ml.
Alkali Scientific CellPro™ Tris-HCl Buffer, 1M, pH 7.4, 1L, 12/cs
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
LAB BIOREAGENTS LowBinding VI Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
Waters Corp Tris CaCl2 Buffer Salts, pH 7.5, 4/pk
Waters Corporation Tris CaCl2 Buffer Salts, pH 7.5, 4/pk
Crescent Chemical Co Inc TE BUFFER (100X) PH 8.0
TE Buffer (100X) pH 8.0 DNase/RNase not detected. 100 x concentrated aqueous solution. Size - 100ML Storage Conditions - +15 °C TO +30 °C Catalog Number - 39799.01
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid purified grade | 60-00-4 | MFCD00003541 | 1kg
Ethylenediaminetetraacetic acid purified grade | Purity: 98.5% | MW: 292.24 | 60-00-4 | MFCD00003541 | 1kg
Bioworld TT - SDS Running Buffer (Anode) (10X), 500 mL
Product DescriptionTris-Tricine (TT)-SDS running buffer has a 10X concentration and a pH of 8.9. It contains 1M Tris-base, 1M Tricine and 1% SDS.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
STEMCELL Technologies EasySep™ Red Blood Cell Lysis Buffer, Size: 10 mL
EasySep™ Red Blood Cell Lysis Buffer is for use as directed with EasySep™ or RoboSep™ Whole Blood kits for the lysis of red blood cells prior to separation from whole blood.